Automated estimation of sodium, potassium, and calcium using the Eppendorf flame photometer

Automated Estimation of Sodium, Potassium, and Calcium Using the Eppendorf Flame Photometer.

An Eppendorf flame photometer may be used to estimate sodium, potassium, and calcium in plasma which has been automatically sampled and diluted. Readings may be automatically recorded by feeding the output from the photomultiplier into a sensitive recorder. This method estimates 60 samples an hour, using approximately 0.12 ml. plasma per estimation. The accuracy of the method is discussed.

Automated estimation of urinary calcium using the Eppendorf flame photometer.

A method is described for estimating urine calcium on a mechanized Eppendorf flame photometer with twin channels at a rate of 60 per hour. Sodium is estimated simultaneously so that a correction can be made for the small sodium error. Errors due to potassium and phosphate are minimized by adding these ions to the standards and to the diluting fluid. Results agree well with those obtained by Tri...

Serum Calcium Using the Eel Flame Photometer

Flame photometry has many applications, and in the clinical field its use in the estimation of serum sodium and potassium is now well established. Difficulties have been encountered in the estimation of serum calcium by flame photometry, since the emission spectrum of calcium is relatively weak and is subject to interference by the presence of other ions, e.g., sodium. Although many methods hav...

Microdetermination of serum calcium using the Eel flame photometer.

Further modification of flame photometer. Dual-channel circuit for convenient determination of sodium and potassium.

S PART OF A CONTINUING PROGRAM in this laboratory directed toward the improvement of clinical laboratory procedures and instruments, additional modifications (1) of the flame photometer have been devised to obtain the benefits of modern components and circuits. Flame photometers using self-generating photocells have proved to be stable and reliable instruments in hundreds of clinical laboratori...